Verticillium Stripe Management

Key Result

This project is still in progress, but is aimed at mitigating the threat posed by verticillium stripe to sustainable canola production through a variety of research activities.

Project Summary

Purpose

The project is designed to mitigate the threat posed by verticillium stripe to sustainable canola production through a variety of research activities. First of all, an evaluation of yield losses associated with verticillium stripe will be important to help farmers and industry predict the impact of this disease and determine whether or not control measures are warranted. Secondly, improved inoculation techniques will facilitate further research into verticillium stripe, allowing researchers to evaluate control measures more efficiently and to more effectively screen genetic material for resistance to the pathogen. Knowledge of the resistance of canola cultivars to this disease will help identify potential management strategies, including an assessment of genetic resistance as a potential management tool.

Verticillium stripe cross-section discoloration comparison (close up)
Verticillium stripe cross-section discoloration; Photo credit: Yixiao (Becky) Wang
Verticillium stripe and blackleg cross-section discoloration comparison (labelled, close up)
Verticillium stripe (L) and blackleg (R) cross-section discoloration comparison; Photo credit: Yixiao (Becky) Wang
Blackleg and verticillium stripe infection and discoloration of canola stem cross-section
Blackleg and verticillium stripe infection and discoloration of canola stem cross-section; Photo credit: Yixiao (Becky) Wang

Objectives

This project includes three specific objectives:

  1. Determine if there is yield loss and measure the extent of potential yield losses from verticillium stripe. This includes:
    • Evaluating the effects of inoculum density on disease intensity.
    • Developing a model to relate disease severity to yield loss.
  2. Investigate disease development. This involves:
    • Determining the effects of inoculation on infection at various canola plant growth stages (using various techniques).
  3. Evaluate canola genotypes (canola cultivars) for resistance to verticillium stripe.

Progress

Greenhouse experiments on the effect of different inoculum densities on plants at different growth stages are being evaluated. Despite COVID-19, field experiments on the effect of different inoculum densities on disease severity and yield were seeded and are being collected.

Control plots of the canola cultivar field assessment comparison
Control plots of the canola cultivar field assessment comparison; Photo credit: Yixiao (Becky) Wang
Inoculated plots of canola cultivar field assessment comparison
Inoculated plots of canola cultivar field assessment comparison; Photo credit: Yixiao (Becky) Wang
Verticillium stripe infection in longitudinal cut of canola stem
Verticillium stripe infection in longitudinal cut of canola stem; Photo credit Yixiao (Becky) Wang
Blackleg infection in longitudinal cut of canola stem;
Blackleg infection in longitudinal cut of canola stem; Photo credit Yixiao (Becky) Wang
Blackleg and verticillium stripe infection in longitudinal cut of canola stem
Blackleg and verticillium stripe infection in longitudinal cut of canola stem; Photo credit Yixiao (Becky) Wang
Blackleg pycnidia on the bottom and verticillium stripe microsclerotia on the top of the infected canola stem (close-up)
Blackleg pycnidia on the bottom and verticillium stripe microsclerotia on the top of the infected canola stem; Photo credit: Yixiao (Becky) Wang