Researchers developed a protocol for a growth chamber and greenhouse-based QR assay which can be used for high-throughput screening to identify canola varieties with quantitative resistance to blackleg disease.
Adult plant resistance (APR), also called quantitative resistance, is the most favourable form of genetic resistance against blackleg disease, since it is controlled by several genes, hence being more durable. Despite its importance, it is very challenging to identify and introduce APR into canola cultivars. The goal is to optimize a protocol for identifying APR to blackleg disease under controlled conditions (growth chamber) and validate results in the field. Molecular markers associated with the APR genes will be developed and offered as a diagnostic and breeding tool.
A growth chamber assay identified 47 lines with APR. A population that consisted of 36 of these lines were tested for their response to blackleg disease in a field trial conducted in Morden, MB. Presence of APR was confirmed under the field conditions, which indicates the validity of the growth-chamber-based assay.
This protocol for growth chamber and greenhouse-based QR assay can be used for high-throughput screening to identify canola varieties with quantitative resistance to blackleg disease.