Defining populations of P. brassicae with near isogenic B. napus lines

Project Summary

Overview

Clubroot disease caused by Plasmodiophora brassicae continues to pose a serious threat to canola production. Genetic resistance can be an effective strategy for clubroot management, but the sources available for resistance to clubroot in Brassica napus are very limited.

This study aims to develop a set of near isogenic B. napus lines containing single clubroot-resistance genes, and to define the populations of P. brassicae with the newly developed near isogenic lines.

Building on previous research, the objectives of this project were to:

• Complete the development of the doubled haploid (DH) lines initiated in a previous project
• Develop a set of near isogenic spring type B. napus lines (NILs) carrying resistance and define populations of P. brassicae collected in western Canada.

Conventional breeding methods such as crossing and backcrossing, and molecular marker-assisted selection (MAS) were used in this project. Next generation sequencing technologies were used for genetic mapping of clubroot resistance (CR) genes, which has greatly accelerated CR gene identification.

Results

Clubroot strains for the study were collected in canola fields in Alberta, Saskatchewan, and Manitoba to define populations of P. brassicae in western Canada. Dr. Yu’s group developed a highly efficient method for testing plants for resistance to clubroot that was used in the study.

Genetic mapping in several DH populations with introgressed CR genes from B. rapa turnips resulted in the identification of four novel genes at the Rcr9 locus and one gene at the Rcr10 locus. They also identified a gene at the RcrM locus from two mapping populations, one from European canola cv. ‘Mendel’ and a second from introgressed B. napus lines originating from turnip cv. ‘Siloga’.

This led to the development of canola DH lines with a broad spectrum of clubroot resistance derived from three sources of turnips. The DH lines, which were distributed to AAFC Clubroot Consortium members in April 2021, showed a high level of resistance to the majority of Canadian races of the clubroot pathogen identified in this study. These lines are very valuable for developing resistant cultivars by canola breeders and for more robust strategies for disease management in the future.

Researchers also developed a set of B. napus near-isogenic lines (NILs) containing single CR genes, which is ideal for differentiating races of P. brassica. This first set of NILs containing eight single CR genes in brassica crops could replace the current Canadian Clubroot Differential set and also be used for monitoring changes in the pathogen populations in canola fields. The NILs can be used for genetic studies on the pathogen such as identification and cloning of the potential Avr genes, developing SNP markers associated with each of the Avr genes and providing information concerning the effectiveness of resistance.

Outcomes

Overall, the project has resulted in the development of canola lines with a broad spectrum of clubroot resistance derived from three sources of turnips. Researchers also developed more than 1200 DH lines for identification of novel resistant genes and produced the first set of near isogenic lines containing eight single CR genes in brassica crops. These lines could replace the current Canadian Clubroot Differential set and monitor race change in the pathogen race structure, potentially revolutionizing current clubroot pathotyping systems. Several breeding lines have been distributed to AAFC Clubroot Consortium members for development of future clubroot resistant canola cultivars.